New 8-OHdG Check ELISA Elisa Kit for 8-hydroxy-2'-deoxyguanosine
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8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.
This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells, we recommend to use 'Highly Sensitive 8-OHdG Check ELISA'.
Content of this kit
| 8-OHdG Microtiter Plate: | Precoated with 8-OHdG(12 X 8wells, split type)* | 1 plate |
| Primary Antibody: | Anti 8-OHdG antibody, powder. | 1 vial |
| Primary Antibody Solution | 1 vial (6mL) | |
| Secondary Antibody: | HRP-anti mouse antibody, powder. | 1 vial |
| Secondary Antibody Solution: | 1 vial (12mL) | |
| Chromatic Solution: | 3,3',5,5'-tetramethylbenzidine | 1 vial (0.25mL) |
| Diluting Solution: | H2O2 containing buffer. | 1 vial (12mL) |
| Washing Solution(5x): | 2 vials (26mL x 2)* | |
| Reaction Terminating Solution: | 1M Phosphoric acid. | 1 vial (12mL) |
| Standard 8-OHdG Solution: | Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL) | 1 vial each |
| Plate Seal: | 2 sheets | |
| *:Trial package (KOG-200TE) contains 32 wells (4 X 8 wells), and one vial of Washing solution(5X). | ||
Specifications
| Assay principle: | Competitive ELISA (detection: 450 nm) |
| Specifity: | Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G). |
| Measuring range: | 0.5 to 200 ng/mL |
| Format: | 96 wells (18 samples in triple assays) |
| Applications: | Urine from human and animals. For serum and tissue samples,Highly Sensitive 8-OHdG Check (code #KOG-HS10E) is recommended. |
| Storage: | Store at 4 - 10°C (don't freeze). |
| Expiry: | 12 months after the day of manufacturing. |
| Required but not provided: | Micropipet and chip (100 micro L, 1000 micro L). Measuring pipet (10 mL, 20 mL)/ measuring cylinders. 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. Microplate reader (filter; 450 nm). |