QuantiChrom Hemoglobin Assay Kit

Cat. No. DIHB-250
Quantity / Size 250
Price Contact support@gentaur.com
Size 250
   
Benefits • Assay kit that are simple, convenient and cost effective • Superior in performance • Researchers need little-to-notime for assay optimization • Specialize for both routine laboratory tests and for high-throughput drug discovery applications • with a focus on safe, non-radioactive assay
Description

Hemoglobin (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration.

Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. BioAssay Systems' QuantiChromTM hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and substantially reduces interference by substances in the raw samples.
Procedure Overview

Procedure using 96-well plate:
1. Blank and Calibrator. Pipette 50 μL water (Blank) and 50 μL Calibrator into wells of a clear bottom 96-well plate. Transfer 200 μL water into the Blank and Calibrator wells. The diluted calibrator is equivalent to 100 mg/dL hemoglobin.
2. Samples. Serum and plasma samples can be assayed directly (n = 1). Blood samples should be diluted 100-fold in distilled water (n = 100). Transfer 50 μL samples into wells (Important: avoid bubble formation during the pipetting steps). Add 200 μL Reagent to sample wells and tap plate lightly to mix.
3. Incubate 5 min at room temperature. Read OD at 390-405nm (peak 400nm).

Procedure using cuvette:
1. Transfer 100 μL sample and 1000 μL Reagent into a cuvet and tap lightly to mix. Read OD 400 nm against water.
2. Transfer 100 μL Calibrator and 1000μL water to cuvet. Read OD at 400nm against water.
Key Features

Key features of our assays include simplicity, high-throughput, sensitivity, accuracy and low interference.

Sensitive and accurate. Linear detection range 0.9 – 200 mg /dL hemoglobin in 96-well plate assay.
Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Safety. Reagents are non-toxic.
Versatility. Assays can be executed in 96-well plate or cuvet.
Notes Storage conditions. The kit is shipped at room temperature. Store reagent and calibrator at 4°C. Shelf life: at least 6 months (see expiry dates on labels). Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.
Protocol Procedure: 5 min.
Storage Temp 2-8°C
Shipping Conditions room temp
References

Thaker PH et al (2006) - Chronic stress promotes tumor growth and angiogenesis in a mouse model of ovarian carcinoma. Nat Med. 12(8):939-44

Liu D et al (2010) - Puma is required for p53-induced depletion of adult stem cells. Nat Cell Biol. 12(10):993-8.

Burne-Taney MJ et al (2006) - Decreased capacity of immune cells to cause tissue injury mediates kidney ischemic preconditioning. J Immunol. 176(11):7015-20

Kasiappan R et al (2009) - Loss of p53 and MCT-1 overexpression synergistically promote chromosome instability and tumorigenicity. Mol Cancer Res. 7(4):536-48

Weis SM et al (2008) - Compensatory role for Pyk2 during angiogenesis in adult mice lacking endothelial cell FAK. J Cell Biol. 181(1):43-50

He Y et al (2009) - Effects of cerebral ischemia on neuronal hemoglobin. J Cereb Blood Flow Metab. 29(3):596-605

Liu W,et al (2009) - Normobaric hyperoxia reduces the neurovascular complications associated with delayed tissue plasminogen activator treatment in a rat model of focal cerebral ischemia. Stroke 40(7):2526-31

Fridén M et al (2010) - Improved measurement of drug exposure in the brain using drug-specific correction for residual blood. J Cereb Blood Flow Metab. 30(1):150-61

Meyer C et al (2010) - Hemodialysis-induced release of hemoglobin limits nitric oxide bioavailability and impairs vascular function. J Am Coll Cardiol. 55(5):454-9

Singleton PA et al (2010) - Methylnaltrexone potentiates the anti-angiogenic effects of mTOR inhibitors. J Angiogenes Res. 2(1):5

French CJ et al (2010) - Vascular rhexis: loss of integrity of coronary vasculature in mice subjected to myocardial infarction. Exp Biol Med (Maywood) 235(8):966-73